Journal article
Cellulose-Microtubule Uncoupling Proteins Prevent Lateral Displacement of Microtubules during Cellulose Synthesis in Arabidopsis
Z Liu, R Schneider, C Kesten, Y Zhang, M Somssich, Y Zhang, AR Fernie, S Persson
Developmental Cell | CELL PRESS | Published : 2016
Abstract
Cellulose is the most abundant biopolymer on Earth and is the major contributor to plant morphogenesis. Cellulose is synthesized by plasma membrane-localized cellulose synthase complexes (CSCs). Nascent cellulose microfibrils become entangled in the cell wall, and further catalysis therefore drives the CSC forward through the membrane: a process guided by cortical microtubules via the protein CSI1/POM2. Still, it is unclear how the microtubules can withstand the forces generated by the motile CSCs to effectively direct CSC movement. Here, we identified a family of microtubule-associated proteins, the cellulose synthase-microtubule uncouplings (CMUs), that located as static puncta along corti..
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Awarded by Australian Research Council
Funding Acknowledgements
Tony Bacic, and Ian Wallace for comments on the manuscript. We thank Prof. Markus Grebe for providing the AVP1 antibody. We thank Drs. Xuna Wu, Witold Szymanski, and Waltraud Shulze for help with mass spectrometry measurements. Z.L. was financially supported by the Chinese Scholarship Council. R.S. and S.P. were supported by the Max-Planck-Gesellschaft and S.P. via an R@MAP Professor grant at University of Melbourne, and the work was supported in part by ARC Discovery grant DP150103495. Youjun Zhang was supported by the Deutsche Akademische Austauchdiesnst (DAAD) and the Max-Planck Gesellschaft; Yi Zhang and A.R.F. were supported solely by the Max-Planck Gesellschaft.